CELL BASED THERAPY

The animal cell culture techniques are used in replacing the damaged and dead cells with normal and healthy cells using the stem cell technology. This therapy is called Cell-Based therapy which involves the use of stem cell technology involving the replacement of damaged and dead cells with normal and healthy cells. This is used to treat blood cancer, and other neuro-degenerative diseases etc.

Equipments Required for Animal Cell Culture

Laminar Flow Cabinets
Depending on the nature of the cells and organisms being handled, tissue culture hoods can be grouped as follows :
a) Class I hoods are found with in specially designed sterile work areas and give good protection to the operator and, to a lesser degree, the cell culture. There is an open front from which the air is drawn over the cell culture and goes out through the top of the hood.
b) Class II hoods offer protection to both operator and the cell culture and is the most common type found in a tissue culture laboratory. The cell culture is protected in a stream of sterile air and the operator is protected from contamination by the inflow of air into the base of the work area.
c) Class III hoods contains a full physical barrier which screens the worker, and is mainly used for working with highly pathogenic organisms.

The Incubators

Generally CO2 incubators are used in animal cell cultures. This is

a) to maintain the sterility of the chamber for which filtered High Efficiency Particulate Air (HEPA) is used.

b) to maintain constant temperature the incubators is made airtight using a silicon gasket on the inner door.

c) to keep an atmosphere with a fixed level of CO2 and high relative humidity which prevents the dessication of the medium and maintains the osmolality?

Inverted Microscope

This type of microscope is used for visualizing cell cultures in situ.

Centrifuges

Only low speed centrifuges are used generally at 20oC to avoid disruption of the separated bands of cells.